First, we isolate the mycelium on Petri dishes and select strains with the highest concentration of valuable bioactive compounds. The mycelium is then transferred into a sterile substrate block — a nutrient medium made from PEFC/FCA-certified Czech oak–beech wood chips. These are the natural tree species on which these mushrooms grow in the wild.
The blocks mature for several weeks until the mycelium fully colonizes the substrate. Temperature, humidity, and sterility are precisely controlled — this is the foundation of stable, high-quality raw material.
This stage reflects a key SIBIPHARM principle: scientific rigor always goes hand in hand with respect for the natural rhythms of mushroom growth.
Cultivation of Organic Fruiting Bodies in Czech Bohemia
Once the substrate is fully colonized, fruiting begins. We cultivate mushrooms in one of the most ecologically clean regions of Czech Bohemia, where forest air and stable natural humidity support the growth of dense, mature, organically-certified fruiting bodies.
Our mycologists carefully control:
🟣 humidity
🟣 temperature
🟣 CO₂ concentration
🟣 light exposure
🟣 air circulation
This precise environmental calibration allows mushrooms to develop fully and form a rich natural biochemical profile.
Chaga, unlike cultivated species, is sourced as wild organic material.
It is harvested in the northern forests of Finnish Lapland — beyond the Arctic Circle. Arctic Chaga grows 15–20 years on birch trees, accumulating exceptionally high levels of natural compounds. A cold climate, short polar summers, and sharp light contrasts make its biochemical profile especially dense and potent.
Drying, Cryo-Milling, and Preservation of the Mushroom’s Natural Structure
After harvesting, fruiting bodies are dried at temperatures no higher than 39 °C until they reach 6–8% residual moisture. This gentle dehydration preserves heat-sensitive natural compounds. For cryo-powder production, we use pharmaceutical-grade milling in liquid nitrogen at –196 °C.
This method:
🟣 prevents oxidation,
🟣 preserves the full spectrum of natural molecules,
🟣 maximizes bioavailability,
🟣 increases the biological activity of the powder.
Cryo-powders are widely used in pharmaceuticals and even in aerospace nutrition, where high nutrient density and minimal volume are essential.
Modern research shows that the effectiveness of mushroom supplements depends not only on the concentration of active compounds but also on their bioavailability. Cryo-milled formats demonstrate significantly higher bioavailability than traditional powders and even extracts.
Ultra-cold processing preserves molecular integrity and prevents oxidation, allowing the body to absorb a wider spectrum of natural compounds. Our chromatographic comparison clearly shows that cryo-powders contain multiple times higher levels of key bioactive fractions than conventional powders.
This confirms: the cryogenic format unlocks more of the mushroom’s natural potential and enhances its practical value.
Results of High-Performance Liquid Chromatography (HPLC) and Atomic Absorption Spectroscopy (AAS) of Chaga
| Structural class | Concentration, mcg% Ground sample | Concentration, mcg% Cryogenic sample, ground |
|---|---|---|
| Amino acids: | ||
| Glycine | not detected | 12.77 |
| Glutamine acid | 1.44 | 14.71 |
| Asparagine acid | 2.06 | 9.35 |
| Leucine | not detected | 7.94 |
| Flavonoids | 3.91 | 7.68 |
| Melanin | 13.42 | 24.22 |
| Calcium | 0.21 | 0.36 |
As a result of the destruction of the chitinous cell wall, the bioavailability of beneficial compounds in cryogenically milled Chaga powder increases manyfold.
Triple Extraction and Ultrasonic Cavitation at 20,000 Hz
For our liquid BOHEMYCO extracts, we use a three-phase extraction system:
🟣 water,
🟣 alcohol (fully removed from the final product),
🟣 glycerin.
A key stage is ultrasonic cavitation at 20,000 Hz, which breaks the chitin cell walls and enables highly efficient extraction of:
🟣 polysaccharides,
🟣 triterpenes,
🟣 phenolic compounds,
🟣 low-molecular fractions,
🟣 both polar and non-polar molecules.
Each mushroom species receives its own extraction protocol — a precise combination of temperature, duration, solvent ratios, and ultrasonic intensity.
This reflects the principle of Yin–Yang: every mushroom has its own “character,” and the task of technology is to reveal it without disrupting its natural harmony.
Quality Control: Scientific Precision and theEuropean Standard of Production
All BOHEMYCO mushroom products are crafted entirely in Europe.
Cultivation takes place in the ecologically pristine region of Czech Bohemia — one of the most environmentally stable areas in Europe.
But purity of origin is only the beginning. True quality is defined by strict control at every production stage.
To ensure the safety and effectiveness of our supplements, we perform multi-level quality control. At every stage — from mycelium formation to the final extract or cryo-powder — the raw material is tested in certified international laboratories. We verify:
🟣 purity and authenticity of raw material,
🟣 absence of microbiological, heavy-metal, or chemical contaminants,
🟣 stability of active fractions,
🟣 compliance with European safety standards.
Quality is also the result of continuous scientific development. This is why we maintain active knowledge exchange with leading institutions:
🟣 Zhejiang University (Hangzhou, China) — one of the world’s largest mycology faculties with a collection of over 3,000 mushroom species;
🟣 Technical University in Zvolen (Slovakia) — mycology and forest bioresource research;
🟣 Czech University of Life Sciences (Prague) — analysis of low-molecular bioactive compounds essential for mushroom efficacy;
🟣 Faculty of Pharmacy, Charles University (Plzeň) — biochemical testing and evaluation of extract properties.
These partnerships allow us to continuously refine our cryo-milling and triple-extraction technologies and to deepen our understanding of each mushroom’s natural potential.
They help us create products that genuinely work — and maintain our position as a leader in the mushroom supplement industry.